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Beads were initially equilibrated in 20 mM Tris-HCl pH 8.0, 300 mM NaCl binding buffer, followed by incubation with 50 M His-tagged Syn constructs (Syn WT or Syn 1103 ) for 45 min at 4 C with gentle rotation

The intronic G 4 C 2 hexanucleotide repeat expansion in the C9orf72 gene is the most common genetic cause of ALS, present in both fALS and sporadic ALS (sALS) cases

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Certainly this class of drugs, both what theyve already done and their potential, is enormous and worthy of a consideration around these large prizes, said Randy Seeley, director of the Michigan Nutrition Obesity Research Center, who has followed the development of these drugs and has consulted for companies making them